A short sequence within <i>Alu</i>Sx induces downstream exon skipping in an <i>ACAT1</i> minigene model

Alu elements, the most abundant retrotransposed elements in human genome, are commonly located introns and affect alternative splicing. We previously showed that an intronic antisense element enhanced splicing a minigene model. The RNA experiment was performed using consisting of exons 9–11 ACAT1 with AluSx inserted into intron 10 pCAGGS vector. Here, we explored sequence affects downstream exon skipping. Our analysis deletion mutants confirmed short 16 bp within right arm resulted findings help clarify mechanism by elements.